SOPs
What is a SOP?Filters
This README file describes how the s-core / s-core+ analysis perl script is to be executed together with data files.
Creators: Eivind Almaas, Marius Eidsaa
Submitter: Eivind Almaas
Metabolic networks with gene expression are researched under very different banners with different techniques. For example, there are the dynamic enzyme-cost Flux Balance Analysis (deFBA) [1], conditional Flux Balance Analysis [2], Metabolism and Expression models (ME models) [3], Resource Balance Analysis [4], etc. At their core, these methods can all understood as Resource Allocation Models (RAM) and while investigating their potential and their results, we encountered the problem of sharing ...
Creators: Henning Lindhorst, Alexandra-M. Reimers
Submitter: Henning Lindhorst
Method extraction of intracellular metabolites in Lactococcus lactis
Creator: Martijn Bekker
Submitter: The JERM Harvester
Method extraction of intracellular metabolites in Lactococcus lactis
Creator: Martijn Bekker
Submitter: The JERM Harvester
Method for transformation of plasmids into Lactococcus lactis
Creator: Martijn Bekker
Submitter: The JERM Harvester
Method for synthesis of LAB-medium sued for the SYSMO-LAB project
Creator: Martijn Bekker
Submitter: The JERM Harvester
Method for analysis of various organic acids in the medium
Creator: Martijn Bekker
Submitter: The JERM Harvester
Creators: Theresa Kouril, Andreas Albersmeier, CeBiTech, University Bielefeld, Germany; Jörn Kalinowski, CeBiTech, University Bielefeld;
Submitter: Theresa Kouril
Protein extraction, iTRAQ labeling, peptides separation, mass spectrometry and data analysis
Creators: Theresa Kouril, Phil Wright, Trong Khoa Pham
Submitter: Theresa Kouril
Creator: Tomas Fiedler
Submitter: Tomas Fiedler
Creator: Tomas Fiedler
Submitter: Tomas Fiedler
Protocol for applying a glucose perturbation in Streptococcus pyogenes.
Creator: Martijn Bekker
Submitter: Martijn Bekker
Perturbation of starved cells with glucose. Concentrations of intra- and extracellular metabolites are followed in time.
Creator: Martijn Bekker
Submitter: Martijn Bekker
Creator: Martijn Bekker
Submitter: The JERM Harvester
This HPLC method uses a isocratic method and a RI detector to identify and quantify almost all excreted catabolic metabolites.
Creator: Martijn Bekker
Submitter: Martijn Bekker
A protocol for acidic quenching of lactic acid bacteria used for analyses of intracellular metabolites.
Creator: Martijn Bekker
Submitter: Martijn Bekker
This protocol for applying glucose perturbations works for Lactococcus lactis and Enterococcus faecalis
Creator: Martijn Bekker
Submitter: Martijn Bekker
Creator: Martijn Bekker
Submitter: The JERM Harvester
Creator: Martijn Bekker
Submitter: The JERM Harvester
Creator: Martijn Bekker
Submitter: The JERM Harvester
Creator: Martijn Bekker
Submitter: The JERM Harvester
Matlab script that reproduces the model redesign results outlined in RobOKoD: microbial strain design for (over)production of target compounds (http://fairdomhub.org/publications/236).
Creator: Natalie Stanford
Submitter: Natalie Stanford
Matlab script that reproduces the model redesign results outlined in RobOKoD: microbial strain design for (over)production of target compounds (http://fairdomhub.org/publications/236) for OptKnock.
Creator: Natalie Stanford
Submitter: Natalie Stanford
Matlab script that reproduces the model redesign results outlined in RobOKoD: microbial strain design for (over)production of target compounds (http://fairdomhub.org/publications/236) for RobustKnock.
Creator: Natalie Stanford
Submitter: Natalie Stanford
SOP for the determination of external metabolites (Glc, Pyr, Gly, Lac) in intact trophozoite incubations, and for the determination of intracellular metabolite concentrations.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for the cultivation conditions of Plasmodium falciparum, including the protocol for synchronisation.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for measurement of G3PDH activity in extracts.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for measurement of ALD activity in extracts.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for measurement of glucose transport activity in intact trophozoites.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk
SOP for measurement of GAPDH activity in extracts.
Creators: Dawie van Niekerk, Jacky Snoep
Submitter: Dawie van Niekerk