Selected Cell
Cell:
Value:
final
Tabelle3
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
R
S
T
U
V
W
X
Y
Z
AA
AB
AC
AD
AE
AF
AG
AH
AI
AJ
AK
AL
AM
AN
AO
AP
AQ
AR
AS
AT
AU
AV
AW
AX
AY
AZ
BA
BB
BC
BD
BE
BF
401
402
403
404
405
406
407
408
409
410
411
412
413
414
415
416
417
418
419
420
421
422
423
424
425
426
427
428
429
430
431
432
433
434
435
436
437
438
439
440
441
442
443
444
445
446
447
448
449
450
451
452
453
454
455
456
457
458
459
460
461
462
463
398 | 9252361 | rat (expressed in Spodoptera frugiperda) | M1; mutant L408T | Ikeda_JBC_vol272_pp20495-501_1997_PYK-M1_mutants | 0.01 - 2.0 | nH | 1.5 | 0.13 | 1.4 | 0.26 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 2 mM ADP, 0.17 mM NADH, 17 U LDH; 1 mL | none>=FBP | nH | ||||||||||||||||||||||||||||||||||||||||||
399 | 9252361 | rat (expressed in Spodoptera frugiperda) | M1; mutant L423C | Ikeda_JBC_vol272_pp20495-501_1997_PYK-M1_mutants | 0.01 - 2.0 | nH | 2.1 | 0.48 | 1.6 | 0.07 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 2 mM ADP, 0.17 mM NADH, 17 U LDH; 1 mL | none>FBP | nH | ||||||||||||||||||||||||||||||||||||||||||
400 | 9252361 | rat (expressed in Spodoptera frugiperda) | M1; mutant L427I | Ikeda_JBC_vol272_pp20495-501_1997_PYK-M1_mutants | 0.01 - 2.0 | nH | 1.4 | 0.01 | 1.2 | 0.14 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 2 mM ADP, 0.17 mM NADH, 17 U LDH; 1 mL | none>FBP | nH | ||||||||||||||||||||||||||||||||||||||||||
401 | 9790887 | Schizosaccharomyces pombe | Nairn_ProtExprPurif_vol14_pp247-53_1998 | 5.6 | nH | 2 | 1 | 6.5 | 25 | 50 mM Mes-TPA [2-(N-morpholino)ethanesulphanatotetrapropylammonium], +/- FBP, 15 mM MgCl2, 100 mM KCl, 0.15 mM NADH, 1.1 units/ml LDH, 2.4 mM ADP | none>FBP | nH | |||||||||||||||||||||||||||||||||||||||||||||
402 | 9790887 | Saccharomyces cerevisiae | Nairn_ProtExprPurif_vol14_pp247-53_1998 | 5.6 | nH | 2.93 | 1.72 | 6.5 | 25 | 50 mM Mes-TPA [2-(N-morpholino)ethanesulphanatotetrapropylammonium], +/- FBP, 15 mM MgCl2, 100 mM KCl, 0.15 mM NADH, 1.1 units/ml LDH, 2.4 mM ADP | none>FBP | nH | |||||||||||||||||||||||||||||||||||||||||||||
403 | 9790887 | Schizosaccharomyces pombe | Nairn_ProtExprPurif_vol14_pp247-53_1998 | 5.6 | S0,5 | 0.78 | 0.52 | 6.5 | 25 | 50 mM Mes-TPA [2-(N-morpholino)ethanesulphanatotetrapropylammonium], +/- FBP, 15 mM MgCl2, 100 mM KCl, 0.15 mM NADH, 1.1 units/ml LDH, 2.4 mM ADP | none>FBP | S0,5 | |||||||||||||||||||||||||||||||||||||||||||||
404 | 9790887 | Saccharomyces cerevisiae | Nairn_ProtExprPurif_vol14_pp247-53_1998 | 5.6 | S0,5 | 2.76 | 0.22 | 6.5 | 25 | 50 mM Mes-TPA [2-(N-morpholino)ethanesulphanatotetrapropylammonium], +/- FBP, 15 mM MgCl2, 100 mM KCl, 0.15 mM NADH, 1.1 units/ml LDH, 2.4 mM ADP | none>FBP | S0,5 | |||||||||||||||||||||||||||||||||||||||||||||
405 | 10734049 | rat (expressed in Spodoptera frugiperda) | M1; WT | Ikeda_JBC_vol275_pp9150-56_2000_mutations | 0.005 - 2 | Vmax/app [µmol/min/mg] | 530 | 540 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 17 U/ml LDH, 0.17 mM NADH | FBP>none | Vmax | ||||||||||||||||||||||||||||||||||||||||||||
406 | 10734049 | rat (expressed in Spodoptera frugiperda) | M1; mutant E432A | Ikeda_JBC_vol275_pp9150-56_2000_mutations | 0.005 - 2 | Vmax/app [µmol/min/mg] | 520 | 490 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 17 U/ml LDH, 0.17 mM NADH | none>FBP | Vmax | ||||||||||||||||||||||||||||||||||||||||||||
407 | 10734049 | rat (expressed in Spodoptera frugiperda) | M1; mutant E432K | Ikeda_JBC_vol275_pp9150-56_2000_mutations | 0.005 - 2 | Vmax/app [µmol/min/mg] | 550 | 530 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 17 U/ml LDH, 0.17 mM NADH | none>FBP | Vmax | ||||||||||||||||||||||||||||||||||||||||||||
408 | 10734049 | rat (expressed in Spodoptera frugiperda) | M1; mutant E432D | Ikeda_JBC_vol275_pp9150-56_2000_mutations | 0.005 - 2 | Vmax/app [µmol/min/mg] | 470 | 510 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 17 U/ml LDH, 0.17 mM NADH | FBP>none | Vmax | ||||||||||||||||||||||||||||||||||||||||||||
409 | 10734049 | rat (expressed in Spodoptera frugiperda) | M1; mutant A398R | Ikeda_JBC_vol275_pp9150-56_2000_mutations | 0.005 - 2 | Vmax/app [µmol/min/mg] | 560 | 590 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 17 U/ml LDH, 0.17 mM NADH | FBP>none | Vmax | ||||||||||||||||||||||||||||||||||||||||||||
410 | 10734049 | rat (expressed in Spodoptera frugiperda) | M1; mutant A398R/E432A | Ikeda_JBC_vol275_pp9150-56_2000_mutations | 0.005 - 2 | Vmax/app [µmol/min/mg] | 560 | 540 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 17 U/ml LDH, 0.17 mM NADH | none>FBP | Vmax | ||||||||||||||||||||||||||||||||||||||||||||
411 | 10734049 | rat (expressed in Spodoptera frugiperda) | M1; mutant A398R/E432K | Ikeda_JBC_vol275_pp9150-56_2000_mutations | 0.005 - 2 | Vmax/app [µmol/min/mg] | 550 | 570 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 17 U/ml LDH, 0.17 mM NADH | FBP>none | Vmax | ||||||||||||||||||||||||||||||||||||||||||||
412 | 10734049 | rat (expressed in Spodoptera frugiperda) | M1; mutant A398R/E432D | Ikeda_JBC_vol275_pp9150-56_2000_mutations | 0.005 - 2 | Vmax/app [µmol/min/mg] | 520 | 500 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 17 U/ml LDH, 0.17 mM NADH | none>FBP | Vmax | ||||||||||||||||||||||||||||||||||||||||||||
413 | 10734049 | rat (expressed in Spodoptera frugiperda) | M1; WT | Ikeda_JBC_vol275_pp9150-56_2000_mutations | 0.005 - 2 | S0,5 [µM] | 58 | 55 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 17 U/ml LDH, 0.17 mM NADH | none>FBP | S0,5 | ||||||||||||||||||||||||||||||||||||||||||||
414 | 10734049 | rat (expressed in Spodoptera frugiperda) | M1; mutant E432A | Ikeda_JBC_vol275_pp9150-56_2000_mutations | 0.005 - 2 | S0,5 [µM] | 66 | 53 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 17 U/ml LDH, 0.17 mM NADH | none>FBP | S0,5 | ||||||||||||||||||||||||||||||||||||||||||||
415 | 10734049 | rat (expressed in Spodoptera frugiperda) | M1; mutant E432K | Ikeda_JBC_vol275_pp9150-56_2000_mutations | 0.005 - 2 | S0,5 [µM] | 70 | 54 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 17 U/ml LDH, 0.17 mM NADH | none>FBP | S0,5 | ||||||||||||||||||||||||||||||||||||||||||||
416 | 10734049 | rat (expressed in Spodoptera frugiperda) | M1; mutant E432D | Ikeda_JBC_vol275_pp9150-56_2000_mutations | 0.005 - 2 | S0,5 [µM] | 74 | 51 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 17 U/ml LDH, 0.17 mM NADH | none>FBP | S0,5 | ||||||||||||||||||||||||||||||||||||||||||||
417 | 10734049 | rat (expressed in Spodoptera frugiperda) | M1; mutant A398R | Ikeda_JBC_vol275_pp9150-56_2000_mutations | 0.005 - 2 | S0,5 [µM] | 390 | 220 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 17 U/ml LDH, 0.17 mM NADH | none>FBP | S0,5 | ||||||||||||||||||||||||||||||||||||||||||||
418 | 10734049 | rat (expressed in Spodoptera frugiperda) | M1; mutant A398R/E432A | Ikeda_JBC_vol275_pp9150-56_2000_mutations | 0.005 - 2 | S0,5 [µM] | 530 | 84 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 17 U/ml LDH, 0.17 mM NADH | none>FBP | S0,5 | ||||||||||||||||||||||||||||||||||||||||||||
419 | 10734049 | rat (expressed in Spodoptera frugiperda) | M1; mutant A398R/E432K | Ikeda_JBC_vol275_pp9150-56_2000_mutations | 0.005 - 2 | S0,5 [µM] | 510 | 77 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 17 U/ml LDH, 0.17 mM NADH | none>FBP | S0,5 | ||||||||||||||||||||||||||||||||||||||||||||
420 | 10734049 | rat (expressed in Spodoptera frugiperda) | M1; mutant A398R/E432D | Ikeda_JBC_vol275_pp9150-56_2000_mutations | 0.005 - 2 | S0,5 [µM] | 370 | 280 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 17 U/ml LDH, 0.17 mM NADH | none>FBP | S0,5 | ||||||||||||||||||||||||||||||||||||||||||||
421 | 10734049 | rat (expressed in Spodoptera frugiperda) | M1; WT | Ikeda_JBC_vol275_pp9150-56_2000_mutations | 0.005 - 2 | nH | 1.2 | 1.2 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 17 U/ml LDH, 0.17 mM NADH | none=FBP | nH | ||||||||||||||||||||||||||||||||||||||||||||
422 | 10734049 | rat (expressed in Spodoptera frugiperda) | M1; mutant E432A | Ikeda_JBC_vol275_pp9150-56_2000_mutations | 0.005 - 2 | nH | 1.3 | 1 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 17 U/ml LDH, 0.17 mM NADH | none>FBP | nH | ||||||||||||||||||||||||||||||||||||||||||||
423 | 10734049 | rat (expressed in Spodoptera frugiperda) | M1; mutant E432K | Ikeda_JBC_vol275_pp9150-56_2000_mutations | 0.005 - 2 | nH | 1.2 | 1.1 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 17 U/ml LDH, 0.17 mM NADH | none>FBP | nH | ||||||||||||||||||||||||||||||||||||||||||||
424 | 10734049 | rat (expressed in Spodoptera frugiperda) | M1; mutant E432D | Ikeda_JBC_vol275_pp9150-56_2000_mutations | 0.005 - 2 | nH | 1 | 1 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 17 U/ml LDH, 0.17 mM NADH | none=FBP | nH | ||||||||||||||||||||||||||||||||||||||||||||
425 | 10734049 | rat (expressed in Spodoptera frugiperda) | M1; mutant A398R | Ikeda_JBC_vol275_pp9150-56_2000_mutations | 0.005 - 2 | nH | 2.7 | 2 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 17 U/ml LDH, 0.17 mM NADH | none>FBP | nH | ||||||||||||||||||||||||||||||||||||||||||||
426 | 10734049 | rat (expressed in Spodoptera frugiperda) | M1; mutant A398R/E432A | Ikeda_JBC_vol275_pp9150-56_2000_mutations | 0.005 - 2 | nH | 1.9 | 1.3 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 17 U/ml LDH, 0.17 mM NADH | none>FBP | nH | ||||||||||||||||||||||||||||||||||||||||||||
427 | 10734049 | rat (expressed in Spodoptera frugiperda) | M1; mutant A398R/E432K | Ikeda_JBC_vol275_pp9150-56_2000_mutations | 0.005 - 2 | nH | 2.5 | 1.3 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 17 U/ml LDH, 0.17 mM NADH | none>FBP | nH | ||||||||||||||||||||||||||||||||||||||||||||
428 | 10734049 | rat (expressed in Spodoptera frugiperda) | M1; mutant A398R/E432D | Ikeda_JBC_vol275_pp9150-56_2000_mutations | 0.005 - 2 | nH | 2.1 | 2.7 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 17 U/ml LDH, 0.17 mM NADH | FBP>none | nH | ||||||||||||||||||||||||||||||||||||||||||||
429 | 20123988 | Leishmania mexicana | WT | Morgan_JBC_vol285_pp12892-8_2010 (+supplemental data! And ref. PMID11943161) | 2.5 | Vmax | 130.1 | 1.1 | 127.4 | 1.2 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | none>F26P | Vmax | ||||||||||||||||||||||||||||||||||||||||||
430 | 20123988 | Leishmania mexicana | mutant K484A | Morgan_JBC_vol285_pp12892-8_2010 (+supplemental data! And ref. PMID11943161) | 2.5 | Vmax | 194.8 | 2.3 | 187.8 | 4 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | none>F26P | Vmax | ||||||||||||||||||||||||||||||||||||||||||
431 | 20123988 | Leishmania mexicana | mutant D482A | Morgan_JBC_vol285_pp12892-8_2010 (+supplemental data! And ref. PMID11943161) | 2.5 | Vmax | 203.9 | 3.5 | 200.2 | 2.2 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | none>=F26P | Vmax | ||||||||||||||||||||||||||||||||||||||||||
432 | 20123988 | Leishmania mexicana | mutant R493A | Morgan_JBC_vol285_pp12892-8_2010 (+supplemental data! And ref. PMID11943161) | 2.5 | Vmax | 139.1 | 3.5 | 138.4 | 2.7 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | none>=F26P | Vmax | ||||||||||||||||||||||||||||||||||||||||||
433 | 20123988 | Leishmania mexicana | mutant E498A | Morgan_JBC_vol285_pp12892-8_2010 (+supplemental data! And ref. PMID11943161) | 2.5 | Vmax | 201.9 | 2.4 | 201.2 | 1.8 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | none>=F26P | Vmax | ||||||||||||||||||||||||||||||||||||||||||
434 | 20123988 | Leishmania mexicana | mutant R493A/E498A | Morgan_JBC_vol285_pp12892-8_2010 (+supplemental data! And ref. PMID11943161) | 2.5 | Vmax | 159.5 | 2.5 | 159.3 | 1.9 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | none>=F26P | Vmax | ||||||||||||||||||||||||||||||||||||||||||
435 | 20123988 | Leishmania mexicana | mutant K484A/D482A | Morgan_JBC_vol285_pp12892-8_2010 (+supplemental data! And ref. PMID11943161) | 2.5 | Vmax | 122.1 | 1.6 | 104.4 | 1.9 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | none>F26P | Vmax | ||||||||||||||||||||||||||||||||||||||||||
436 | 20123988 | Leishmania mexicana | WT | Morgan_JBC_vol285_pp12892-8_2010 (+supplemental data! And ref. PMID11943161) | 2.5 | S0,5 | 0.411 | 0.006 | 0.053 | 0.002 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | none>F26P | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
437 | 20123988 | Leishmania mexicana | mutant K484A | Morgan_JBC_vol285_pp12892-8_2010 (+supplemental data! And ref. PMID11943161) | 2.5 | S0,5 | 0.524 | 0.01 | 0.137 | 0.007 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | none>F26P | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
438 | 20123988 | Leishmania mexicana | mutant D482A | Morgan_JBC_vol285_pp12892-8_2010 (+supplemental data! And ref. PMID11943161) | 2.5 | S0,5 | 0.802 | 0.026 | 0.663 | 0.016 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | none>F26P | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
439 | 20123988 | Leishmania mexicana | mutant R493A | Morgan_JBC_vol285_pp12892-8_2010 (+supplemental data! And ref. PMID11943161) | 2.5 | S0,5 | 0.687 | 0.023 | 0.439 | 0.013 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | none>F26P | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
440 | 20123988 | Leishmania mexicana | mutant E498A | Morgan_JBC_vol285_pp12892-8_2010 (+supplemental data! And ref. PMID11943161) | 2.5 | S0,5 | 0.148 | 0.003 | 0.051 | 0.015 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | F26P>none | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
441 | 20123988 | Leishmania mexicana | mutant R493A/E498A | Morgan_JBC_vol285_pp12892-8_2010 (+supplemental data! And ref. PMID11943161) | 2.5 | S0,5 | 0.439 | 0.017 | 0.369 | 0.01 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | none>F26P | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
442 | 20123988 | Leishmania mexicana | mutant K484A/D482A | Morgan_JBC_vol285_pp12892-8_2010 (+supplemental data! And ref. PMID11943161) | 2.5 | S0,5 | 0.841 | 0.018 | 0.73 | 0.031 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | none>F26P | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
443 | 20123988 | Leishmania mexicana | WT | Morgan_JBC_vol285_pp12892-8_2010 (+supplemental data! And ref. PMID11943161) | 2.5 | nH | 2.45 | 0.08 | 1.27 | 0.05 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | none>F26P | nH | ||||||||||||||||||||||||||||||||||||||||||
444 | 20123988 | Leishmania mexicana | mutant K484A | Morgan_JBC_vol285_pp12892-8_2010 (+supplemental data! And ref. PMID11943161) | 2.5 | nH | 2.16 | 0.07 | 1.58 | 0.12 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | none>F26P | nH | ||||||||||||||||||||||||||||||||||||||||||
445 | 20123988 | Leishmania mexicana | mutant D482A | Morgan_JBC_vol285_pp12892-8_2010 (+supplemental data! And ref. PMID11943161) | 2.5 | nH | 1.9 | 0.08 | 1.6 | 0.05 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | none>F26P | nH | ||||||||||||||||||||||||||||||||||||||||||
446 | 20123988 | Leishmania mexicana | mutant R493A | Morgan_JBC_vol285_pp12892-8_2010 (+supplemental data! And ref. PMID11943161) | 2.5 | nH | 3.14 | 0.27 | 2.58 | 0.18 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | none>F26P | nH | ||||||||||||||||||||||||||||||||||||||||||
447 | 20123988 | Leishmania mexicana | mutant E498A | Morgan_JBC_vol285_pp12892-8_2010 (+supplemental data! And ref. PMID11943161) | 2.5 | nH | 2.42 | 0.11 | 1.45 | 0.04 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | none>F26P | nH | ||||||||||||||||||||||||||||||||||||||||||
448 | 20123988 | Leishmania mexicana | mutant R493A/E498A | Morgan_JBC_vol285_pp12892-8_2010 (+supplemental data! And ref. PMID11943161) | 2.5 | nH | 1.38 | 0.05 | 1.49 | 0.05 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | F26P>none | nH | ||||||||||||||||||||||||||||||||||||||||||
449 | 20123988 | Leishmania mexicana | mutant K484A/D482A | Morgan_JBC_vol285_pp12892-8_2010 (+supplemental data! And ref. PMID11943161) | 2.5 | nH | 2.06 | 0.07 | 1.79 | 0.1 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | none>F26P | nH | ||||||||||||||||||||||||||||||||||||||||||
450 | 11943161 | Leishmania mexicana | WT, expressed in E. coli BL21(DE3) cells | Hannaert_FEBSlett_vol514_2002_pp255-9_Ref+zu+20123988 | 2.5 | S0,5 | 2.67 | 0.03 | 0.57 | 0.02 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | none>F26P | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
451 | 11943161 | Leishmania mexicana | mutant K453E, expressed in E. coli BL21(DE3) cells | Hannaert_FEBSlett_vol514_2002_pp255-9_Ref+zu+20123988 | 2.5 | S0,5 | 2.37 | 0.07 | 1.96 | 0.05 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | none>F26P | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
452 | 11943161 | Leishmania mexicana | mutant K453Q, expressed in E. coli BL21(DE3) cells | Hannaert_FEBSlett_vol514_2002_pp255-9_Ref+zu+20123988 | 2.5 | S0,5 | 2.33 | 0.09 | 2.45 | 0.08 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | F26P>none | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
453 | 11943161 | Leishmania mexicana | mutant H480L, expressed in E. coli BL21(DE3) cells | Hannaert_FEBSlett_vol514_2002_pp255-9_Ref+zu+20123988 | 2.5 | S0,5 | 3.56 | 0.08 | 3.24 | 0.1 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | none>F26P | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
454 | 11943161 | Leishmania mexicana | mutant H480Q, expressed in E. coli BL21(DE3) cells | Hannaert_FEBSlett_vol514_2002_pp255-9_Ref+zu+20123988 | 2.5 | S0,5 | 2.57 | 0.1 | 2.41 | 0.06 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | none>F26P | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
455 | 11943161 | Leishmania mexicana | WT, expressed in E. coli BL21(DE3) cells | Hannaert_FEBSlett_vol514_2002_pp255-9_Ref+zu+20123988 | 2.5 | nH | 1.59 | 1.26 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | none>F26P | nH | ||||||||||||||||||||||||||||||||||||||||||||
456 | 11943161 | Leishmania mexicana | mutant K453E, expressed in E. coli BL21(DE3) cells | Hannaert_FEBSlett_vol514_2002_pp255-9_Ref+zu+20123988 | 2.5 | nH | 1.7 | 1.88 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | F26P>none | nH | ||||||||||||||||||||||||||||||||||||||||||||
457 | 11943161 | Leishmania mexicana | mutant K453Q, expressed in E. coli BL21(DE3) cells | Hannaert_FEBSlett_vol514_2002_pp255-9_Ref+zu+20123988 | 2.5 | nH | 1.54 | 1.5 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | none>F26P | nH | ||||||||||||||||||||||||||||||||||||||||||||
458 | 11943161 | Leishmania mexicana | mutant H480L, expressed in E. coli BL21(DE3) cells | Hannaert_FEBSlett_vol514_2002_pp255-9_Ref+zu+20123988 | 2.5 | nH | 1.85 | 1.74 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | none>F26P | nH | ||||||||||||||||||||||||||||||||||||||||||||
459 | 11943161 | Leishmania mexicana | mutant H480Q, expressed in E. coli BL21(DE3) cells | Hannaert_FEBSlett_vol514_2002_pp255-9_Ref+zu+20123988 | 2.5 | nH | 1.61 | 1.59 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, 1.6 U of beef heart LDH; 1 mL | none>F26P | nH | ||||||||||||||||||||||||||||||||||||||||||||
A
B
C
D
E
F
G
H
I
J
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35