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Metadata | Values (examples) | ||||||||
Asset Title | LDH Kinetic Data | ||||||||
Uploader | Dawie van Niekerk | ||||||||
Uploader SEEK ID | |||||||||
Project | |||||||||
ASSAY | |||||||||
Assay SEEK ID | 0 | ||||||||
Assay Title | LDH | ||||||||
Assay_type | enzymaticAssay | ||||||||
Technology_type | initial rate experiment | ||||||||
Description | Kinetic characterisation of LDH. Experimental data for enzyme reaction rates. | ||||||||
Experimentalist | Gerald Penkler | ||||||||
Date | |||||||||
SOP | |||||||||
Publication (optional) | |||||||||
Experimental_conditions | |||||||||
Item | temperature | pH | Buffer | Buffer | Buffer | Buffer | |||
Compound (if concentration) | HEPES | MgCl | KCl | NaCl | |||||
Unit | °C | mM | mM | mM | mM | ||||
Start_value (optional) | 37 | 7.17 | 20 | 20 | 10 | 20 | |||
End_value (optional) | 37 | ||||||||
Comments | |||||||||
Culture growth | Batch | ||||||||
FACTORS_STUDIED | |||||||||
Item | concentration | concentration | concentration | concentration | |||||
Compound (if concentration) | PYR | NADH | NAD | LAC | |||||
Unit | mM | mM | mM | mM | |||||
Start_value (optional) | 0 | 0 | 0 | 0 | |||||
End_value (optional) | 2.5 | 0.8 | 2 | 80 | |||||
SD (optional) | |||||||||
Comments | |||||||||
ENZYME IDENTITY | |||||||||
Enzyme Name (IUBMB) | L-lactate dehydrogenase | ||||||||
EC Number | 1.1.1.27 | ||||||||
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20081208 | 0 | 0 | 0.5 | 20 | -1.156446219 | 1.83231 | -0.6311411382353422 | 0.542612 | 0.290792 | -5.138862228352486 | ||
20081208 | 0 | 0 | 0.5 | 20 | -1.122734558 | 1.83231 | -0.6127426898286862 | 0.542612 | 0.290792 | -4.989058823298572 | ||
20081208 | 0 | 0 | 0.4 | 20 | -1.114402653 | 1.83231 | -0.608195476202171 | -0.5506248324064523 | 0.06367757104517811 | 0.542612 | 0.290792 | -4.9520346096418875 |
20081208 | 0 | 0 | 0.4 | 20 | -0.775908253 | 1.83231 | -0.4234590505973334 | 0.542612 | 0.290792 | -3.4478781187563934 | ||
20081208 | 0 | 0 | 0.4 | 20 | -1.136435254 | 1.83231 | -0.6202199704198526 | 0.542612 | 0.290792 | -5.049940157873233 | ||
20081208 | 0 | 0 | 0.075 | 20 | -0.099730532 | 1.83231 | -0.05442885319623862 | -0.16875571218843968 | 0.0730683585086851 | 0.542612 | 0.290792 | -0.4431693022019374 |
20081208 | 0 | 0 | 0.075 | 20 | -0.558392728 | 1.83231 | -0.30474795640475677 | 0.542612 | 0.290792 | -2.481311496687857 | ||
20081208 | 0 | 0 | 0.075 | 20 | -0.269515077 | 1.83231 | -0.1470903269643237 | 0.542612 | 0.290792 | -1.1976353300410691 | ||
20081208 | 0 | 0 | 0.025 | 20 | -0.294230373 | 1.83231 | -0.1605789266008481 | -0.11151828711662691 | 0.03518613878544697 | 0.542612 | 0.290792 | -1.3074618822751871 |
20081208 | 0 | 0 | 0.025 | 20 | -0.079331792 | 1.83231 | -0.04329605361538167 | 0.542612 | 0.290792 | -0.3525240886418739 | ||
20081208 | 0 | 0 | 0.025 | 20 | -0.239446053 | 1.83231 | -0.13067988113365095 | 0.542612 | 0.290792 | -1.0640185918492655 | ||
20081208 | 0 | 0 | 0.0125 | 20 | -0.113050788 | 1.83231 | -0.061698505165610616 | -0.12383650874215242 | 0.043116841122228196 | 0.542612 | 0.290792 | -0.5023600879953107 |
20081208 | 0 | 0 | 0.0125 | 20 | -0.378714917 | 1.83231 | -0.20668714191375911 | 0.542612 | 0.290792 | -1.6828830863988036 | ||
20081208 | 0 | 0 | 0.0125 | 20 | -0.188954915 | 1.83231 | -0.10312387914708755 | 0.542612 | 0.290792 | -0.8396527738183167 | ||
20080530 | 0 | 0 | 0.6 | 20 | -0.809365336158573 | 1.7148 | -0.4719881829709429 | -0.5768406132349932 | 0.1048524302640502 | 0.542612 | 0.290792 | -3.84301085566962 |
20080530 | 0 | 0 | 0.6 | 20 | -1.16896723099216 | 1.7148 | -0.6816930434990436 | 0.542612 | 0.290792 | -5.550464738144869 | ||
20080530 | 0 | 0 | 0.36 | 20 | -1.27303947827728 | 1.7148 | -0.742383647234243 | -0.742383647234243 | 0 | 0.542612 | 0.290792 | -6.044618315303119 |
20080530 | 0 | 0 | 0.24 | 20 | -0.773262219057958 | 1.7148 | -0.45093434747956496 | -0.45093434747956496 | 0 | 0.542612 | 0.290792 | -3.6715868216237744 |
20080530 | 0 | 0 | 0.192 | 20 | -0.733748479539131 | 1.7148 | -0.42789157892414914 | -0.42789157892414914 | 0 | 0.542612 | 0.290792 | -3.483968544518313 |
20080530 | 0 | 0 | 0.144 | 20 | -0.64552414012711 | 1.7148 | -0.37644281556281195 | -0.37644281556281195 | 0 | 0.542612 | 0.290792 | -3.065063658247954 |
20080530 | 0 | 0 | 0.096 | 20 | -0.577832826839204 | 1.7148 | -0.33696805857196405 | -0.33696805857196405 | 0 | 0.542612 | 0.290792 | -2.74365323927124 |
20080530 | 0 | 0 | 0.072 | 20 | -0.372045787020595 | 1.7148 | -0.21696162060916432 | -0.21696162060916432 | 0 | 0.542612 | 0.290792 | -1.7665397002450431 |
20080530 | 0 | 0 | 0.048 | 20 | -0.17132738326149 | 1.7148 | -0.09991100026912175 | -0.09991100026912175 | 0 | 0.542612 | 0.290792 | -0.8134929485272366 |
20080530 | 0 | 0 | 0.036 | 20 | -0.0236227962371186 | 1.7148 | -0.013775831722135876 | -0.013775831722135876 | 0 | 0.542612 | 0.290792 | -0.1121652464280134 |
20110920 | 0 | 0 | 1 | 20 | -1.116801516 | 1.54853 | -0.7212010848998728 | -0.7736976158542842 | 0.09018803378327687 | 0.542612 | 0.290792 | -5.872146164645707 |
20110920 | 0 | 0 | 1 | 20 | -1.2271029 | 1.54853 | -0.7924308214887668 | 0.542612 | 0.290792 | -6.452111216386121 | ||
20110920 | 0 | 0 | 1 | 20 | -0.937561766 | 1.54853 | -0.6054527622971463 | 0.542612 | 0.290792 | -4.9297029503095295 | ||
20110920 | 0 | 0 | 0.5 | 20 | -1.061650824 | 1.54853 | -0.6855862166054258 | -0.509509656261503 | 0.04577053995813402 | 0.542612 | 0.290792 | -5.5821636387755 |
20110920 | 0 | 0 | 0.5 | 20 | -0.882411074 | 1.54853 | -0.5698378940026994 | 0.542612 | 0.290792 | -4.639720424439322 | ||
20110920 | 0 | 0 | 0.5 | 20 | -0.785897363 | 1.54853 | -0.5075118744874171 | 0.542612 | 0.290792 | -4.13225100416646 | ||
20110920 | 0 | 0 | 0.25 | 20 | -0.785897363 | 1.54853 | -0.5075118744874171 | -0.4182886782632605 | 0.044468018515185316 | 0.542612 | 0.290792 | -4.13225100416646 |
20110920 | 0 | 0 | 0.25 | 20 | -0.689383652 | 1.54853 | -0.44518585497213486 | 0.542612 | 0.290792 | -3.6247815838935966 | ||
20110920 | 0 | 0 | 0.25 | 20 | -0.510143902 | 1.54853 | -0.3294375323694084 | 0.542612 | 0.290792 | -2.6823383695574208 | ||
20110920 | 0 | 0 | 0.125 | 20 | -0.606657614 | 1.54853 | -0.39176355253046435 | -0.28634999370848724 | 0.03691931946152267 | 0.542612 | 0.290792 | -3.1898077950882864 |
20110920 | 0 | 0 | 0.125 | 20 | -0.468780883 | 1.54853 | -0.30272638114857314 | 0.542612 | 0.290792 | -2.4648514751547648 | ||
20110920 | 0 | 0 | 0.125 | 20 | -0.372267172 | 1.54853 | -0.24040036163329093 | 0.542612 | 0.290792 | -1.9573820548819023 | ||
20110920 | 0 | 0 | 0.0625 | 20 | -0.358479499 | 1.54853 | -0.23149664455967917 | -0.19683960994029723 | 0.01881537883215512 | 0.542612 | 0.290792 | -1.8848864234143503 |
20110920 | 0 | 0 | 0.0625 | 20 | -0.330904153 | 1.54853 | -0.21368921041245567 | 0.542612 | 0.290792 | -1.7398951604792465 | ||
20110920 | 0 | 0 | 0.0625 | 20 | -0.193027422 | 1.54853 | -0.12465203903056447 | 0.542612 | 0.290792 | -1.0149388405457254 | ||
20110920 | 0 | 0 | 0.03125 | 20 | -0.248178115 | 1.54853 | -0.16026690797078522 | -0.1342522911948674 | 0.014382416941398166 | 0.542612 | 0.290792 | -1.304921371673936 |
20110920 | 0 | 0 | 0.03125 | 20 | -0.193027422 | 1.54853 | -0.12465203903056447 | 0.542612 | 0.290792 | -1.0149388405457254 | ||
20110920 | 0 | 0 | 0.03125 | 20 | -0.151664403 | 1.54853 | -0.09794088780972923 | 0.542612 | 0.290792 | -0.7974519461430699 | ||
20110920 | 0 | 0 | 0.015625 | 20 | -0.123104224 | 1.54853 | -0.07949747437892711 | -0.07101774349436778 | 0.01353274408855789 | 0.542612 | 0.290792 | -0.6472824279487153 |
20110920 | 0 | 0 | 0.015625 | 20 | -0.13787673 | 1.54853 | -0.08903717073611749 | 0.542612 | 0.290792 | -0.7249563146755181 | ||
20110920 | 0 | 0 | 0.015625 | 20 | -0.068938365 | 1.54853 | -0.04451858536805874 | 0.542612 | 0.290792 | -0.36247815733775907 | ||
20110920 | 0 | 0 | 2 | 20 | -1.089747353 | 1.49769 | -0.7276187682364174 | -0.6756459991720583 | 0.051972769064359096 | 0.542612 | 0.290792 | -5.92440007188412 |
20110920 | 0 | 0 | 2 | 20 | -0.93406916 | 1.49769 | -0.6236732301076992 | 0.542612 | 0.290792 | -5.0780572060253855 | ||
20110920 | 0 | 0 | 1 | 20 | -0.957420889 | 1.49769 | -0.6392650608603917 | 0.542612 | 0.290792 | -5.205008636176021 | ||
20110920 | 0 | 0 | 1 | 20 | -1.662643105 | 1.49769 | -1.1101383497252435 | 0.542612 | 0.290792 | -9.038941827812485 | ||
20110920 | 0 | 0 | 0.5 | 20 | -0.537089767 | 1.49769 | -0.35861210731192705 | 0.542612 | 0.290792 | -2.919882893464597 | ||
20110920 | 0 | 0 | 0.5 | 20 | -0.747255328 | 1.49769 | -0.4989385840861594 | 0.542612 | 0.290792 | -4.062445764820309 | ||
20110920 | 0 | 0 | 0.5 | 20 | -0.653848412 | 1.49769 | -0.43657126107538946 | 0.542612 | 0.290792 | -3.55464004421777 | ||
20110920 | 0 | 0 | 0.25 | 20 | -0.864013973 | 1.49769 | -0.5768977378496218 | 0.542612 | 0.290792 | -4.697202915573483 | ||
20110920 | 0 | 0 | 0.25 | 20 | -0.468591362 | 1.49769 | -0.31287607048187543 | 0.542612 | 0.290792 | -2.5474920320518346 | ||
20110920 | 0 | 0 | 0.25 | 20 | -0.505954128 | 1.49769 | -0.3378229994191054 | 0.542612 | 0.290792 | -2.7506143181182536 | ||
20110920 | 0 | 0 | 0.125 | 20 | -0.261539365 | 1.49769 | -0.17462850456369478 | 0.542612 | 0.290792 | -1.421856018774406 | ||
20110920 | 0 | 0 | 0.125 | 20 | -0.482602399 | 1.49769 | -0.32223116866641294 | 0.542612 | 0.290792 | -2.6236628879676194 | ||
20110920 | 0 | 0 | 0.0625 | 20 | -0.359616627 | 1.49769 | -0.24011419385854219 | 0.542612 | 0.290792 | -1.9550520264943696 | ||
20110920 | 0 | 0 | 0.0625 | 20 | -0.272436838 | 1.49769 | -0.18190469189218064 | 0.542612 | 0.290792 | -1.4811000166119077 | ||
20110920 | 0 | 0 | 0.0625 | 20 | -0.283334312 | 1.49769 | -0.1891808798883614 | 0.542612 | 0.290792 | -1.5403440198858989 | ||
20110920 | 0 | 0 | 0.03125 | 20 | -0.261539365 | 1.49769 | -0.17462850456369478 | 0.542612 | 0.290792 | -1.421856018774406 | ||
20110920 | 0 | 0 | 0.03125 | 20 | -0.170396859 | 1.49769 | -0.11377311659956334 | 0.542612 | 0.290792 | -0.9263607394221662 | ||
0 | 0 | 2 | 0 | 0 | 1.49041 | 0 | 0 | 0 | 0.542612 | 0.290792 | 0 | |
20081208 | 0 | 0 | 2 | 80 | -1.068034854 | 1.49041 | -0.7166047289001013 | -0.8153858935908017 | 0.04941869460959318 | 0.542612 | 0.290792 | -5.834721825136911 |
20081208 | 0 | 0 | 2 | 80 | -1.293173931 | 1.49041 | -0.8676632141491268 | 0.542612 | 0.290792 | -7.0646665983278805 | ||
20081208 | 0 | 0 | 2 | 80 | -1.284569084 | 1.49041 | -0.8618897377231768 | 0.542612 | 0.290792 | -7.017657937135945 | ||
20081208 | 0 | 0 | 2 | 30 | -1.349052627 | 1.49041 | -0.9051553780503352 | -0.8529732746917515 | 0.03416004888096099 | 0.542612 | 0.290792 | -7.369934395432365 |
20081208 | 0 | 0 | 2 | 30 | -1.289312717 | 1.49041 | -0.8650725082359888 | 0.542612 | 0.290792 | -7.04357261481887 | ||
20081208 | 0 | 0 | 2 | 30 | -1.175474351 | 1.49041 | -0.7886919377889307 | 0.542612 | 0.290792 | -6.421668567258524 | ||
20081208 | 0 | 0 | 2 | 5 | -0.932918596 | 1.49041 | -0.6259476224663012 | -0.6698400247806532 | 0.02234902145317624 | 0.542612 | 0.290792 | -5.096575708902178 |
20081208 | 0 | 0 | 2 | 5 | -1.020140741 | 1.49041 | -0.6844698713776747 | 0.542612 | 0.290792 | -5.573074159454389 | ||
20081208 | 0 | 0 | 2 | 5 | -1.041949477 | 1.49041 | -0.6991025804979838 | 0.542612 | 0.290792 | -5.692216252468752 | ||
20081208 | 0 | 0 | 2 | 2.5 | -0.765787343 | 1.49041 | -0.5138098529934716 | -0.4570129798735471 | 0.04157929123305125 | 0.542612 | 0.290792 | -4.183530253606973 |
20081208 | 0 | 0 | 2 | 2.5 | -0.560411036 | 1.49041 | -0.3760113230587556 | 0.542612 | 0.290792 | -3.0615503703372466 | ||
20081208 | 0 | 0 | 2 | 2.5 | -0.717211767 | 1.49041 | -0.48121776356841406 | 0.542612 | 0.290792 | -3.9181597253004155 | ||
20081208 | 0 | 0 | 2 | 1.25 | -0.332079208 | 1.49041 | -0.2228106413671406 | -0.22089817611708637 | 0.0027818904845422127 | 0.542612 | 0.290792 | -1.8141634566841396 |
20081208 | 0 | 0 | 2 | 1.25 | -0.3210599 | 1.49041 | -0.2154171670882509 | 0.542612 | 0.290792 | -1.7539644878539462 | ||
20081208 | 0 | 0 | 2 | 1.25 | -0.334547444 | 1.49041 | -0.22446671989586758 | 0.542612 | 0.290792 | -1.8276475395348553 | ||
20081208 | 0 | 0 | 2 | 0.625 | -0.313828097 | 1.49041 | -0.210564943203548 | -0.18364129534825988 | 0.015799939779116555 | 0.542612 | 0.290792 | -1.7144568269932914 |
20081208 | 0 | 0 | 2 | 0.625 | -0.232284641 | 1.49041 | -0.15585284653216228 | 0.542612 | 0.290792 | -1.268981306565855 | ||
20081208 | 0 | 0 | 2 | 0.625 | -0.274989731 | 1.49041 | -0.18450609630906933 | 0.542612 | 0.290792 | -1.5022811092213928 | ||
20110920 | 0 | 0 | 2 | 20 | -0.684971596344265 | 0.803091 | -0.8529190295299848 | -0.7903325447568376 | 0.03882632385249309 | 0.542612 | 0.290792 | -6.9446168521823335 |
20110920 | 0 | 0 | 2 | 20 | -0.587118511152227 | 0.803091 | -0.731073453882844 | 0.542612 | 0.290792 | -5.9525287304419985 | ||
20110920 | 0 | 0 | 2 | 20 | -0.550876627747766 | 0.803091 | -0.6859454629024183 | 0.542612 | 0.290792 | -5.585088685352961 | ||
20110920 | 0 | 0 | 2 | 10 | -0.46027191923662 | 0.803091 | -0.5731254854513622 | -0.578840368305626 | 0.02865055136106885 | 0.542612 | 0.290792 | -4.666488572630431 |
20110920 | 0 | 0 | 2 | 10 | -0.40953328247038 | 0.803091 | -0.5099462980787731 | 0.542612 | 0.290792 | -4.152072509505833 | ||
20110920 | 0 | 0 | 2 | 10 | -0.427654224172608 | 0.803091 | -0.5325102935689828 | 0.542612 | 0.290792 | -4.335792532050326 | ||
20110920 | 0 | 0 | 2 | 5 | -0.333425327321016 | 0.803091 | -0.4151775170198844 | -0.4376039100622382 | 0.02775296304772018 | 0.542612 | 0.290792 | -3.3804484148188942 |
20110920 | 0 | 0 | 2 | 5 | -0.311680197278341 | 0.803091 | -0.3881007224316311 | 0.542612 | 0.290792 | -3.159984387765488 | ||
20110920 | 0 | 0 | 2 | 5 | -0.282686690554774 | 0.803091 | -0.3519983296472928 | 0.542612 | 0.290792 | -2.8660323516942756 | ||
20110920 | 0 | 0 | 2 | 2.5 | -0.242820618809871 | 0.803091 | -0.30235753956882966 | -0.32501540142792756 | 0.03420793744737984 | 0.542612 | 0.290792 | -2.4618483020963753 |
20110920 | 0 | 0 | 2 | 2.5 | -0.221075488767196 | 0.803091 | -0.2752807449805763 | 0.542612 | 0.290792 | -2.241384275042969 | ||
20110920 | 0 | 0 | 2 | 2.5 | -0.184833605362737 | 0.803091 | -0.23015275400015314 | 0.542612 | 0.290792 | -1.8739442299539508 | ||
20110920 | 0 | 0 | 2 | 1.25 | -0.202954547064966 | 0.803091 | -0.2527167494903641 | -0.23284840404207255 | 0.011954412629051466 | 0.542612 | 0.290792 | -2.057664252498455 |
20110920 | 0 | 0 | 2 | 1.25 | -0.15584009863917 | 0.803091 | -0.1940503612158149 | 0.542612 | 0.290792 | -1.579992193882739 | ||
20110920 | 0 | 0 | 2 | 1.25 | -0.173961040341399 | 0.803091 | -0.21661435670602586 | 0.542612 | 0.290792 | -1.7637122164272427 | ||
20110920 | 0 | 0 | 2 | 0.625 | -0.159464286979617 | 0.803091 | -0.1985631603138586 | -0.19328367603213661 | 0.02149794132966133 | 0.542612 | 0.290792 | -1.616736198391652 |
20110920 | 0 | 0 | 2 | 0.625 | -0.108725650213374 | 0.803091 | -0.1353839729412657 | 0.542612 | 0.290792 | -1.1023201352670229 | ||
20110920 | 0 | 0 | 2 | 0.625 | -0.126846591915601 | 0.803091 | -0.15794796843147413 | 0.542612 | 0.290792 | -1.2860401578115064 | ||
20110920 | 0 | 0 | 2 | 0.3125 | -0.126846591915604 | 0.803091 | -0.15794796843147788 | -0.12629422774453655 | 0.009161077986257662 | 0.542612 | 0.290792 | -1.2860401578115368 |
20110920 | 0 | 0 | 2 | 0.3125 | -0.119598215234712 | 0.803091 | -0.14892237023539298 | 0.542612 | 0.290792 | -1.212552148793731 | ||
20110920 | 0 | 0 | 2 | 0.3125 | -0.0906047085111455 | 0.803091 | -0.11281997745105535 | 0.542612 | 0.290792 | -0.9186001127225241 | ||
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20110920 | 0 | 0 | 2 | 10 | -0.416781659 | 0.618678 | -0.6736649096945423 | 0.542612 | 0.290792 | -5.485098259757069 | ||
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