Growth of Fei-0 and Ler plants in 12hL:12hD
Data for Figure 3A-3F and Supplementary Figures 2, 3, and 6, including leaf number, biomass and leaf areas. Image data for leaf areas are included in a .ZIP archive. The 'Summary' sheets in the XLSX files often include published graphs. Simulation data are included from FMv1. These data were acquired in June 2012. Experimental conditions: ~22C constant temperature; 12:12-h light/dark cycle; light intensity = 130 μmol·m−2·s−1; average daytime CO2 concentration = 375 ppm. 10 plants per genotype per timepoint measured for shoot biomass, (error bars showed the SEs) plants for total shoot biomass and 5 plants for individual leaf biomass. 24 plants for leat number (error bars showed the SDs). Biomass and image/area data collected from plants at 18, 25 and 30 days after sowing.
SEEK ID: https://fairdomhub.org/assays/714
Experimental assay
Projects: Millar group
Investigation: Arabidopsis Framework Model v1, predicting rosette growth with a multi-model ensemble
Study: Test of FMv1, growth study of other accessions and transgenic line in 12L:12D
Assay position:
Assay type: Cultivation Experiment
Technology type: Cultivation experiment
Organisms: Arabidopsis thaliana, Arabidopsis thaliana : Arabidopsis accession Fei-0 (wild-type / wild-type)
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Created: 22nd Aug 2018 at 13:54
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Projects: Millar group, TiMet
Institutions: University of Edinburgh
https://orcid.org/0000-0002-9258-583X
Projects: Millar group, TiMet, PHYTOCAL: Phytochrome Control of Resource Allocation and Growth in Arabidopsis and in Brassicaceae crops, POP - the Parameter Optimisation Problem, Regulation of flowering time in natural conditions, PlaSMo model repository
Institutions: University of Edinburgh
https://orcid.org/0000-0003-1756-3654
SynthSys is the University of Edinburgh's research organisation in interdisciplinary, Synthetic and Systems Biology, founded in 2012 as the successor to the Centre for Systems Biology at Edinburgh (CSBE).
Projects: Millar group, PHYTOCAL: Phytochrome Control of Resource Allocation and Growth in Arabidopsis and in Brassicaceae crops, TiMet, POP - the Parameter Optimisation Problem, Regulation of flowering time in natural conditions, PlaSMo model repository
Web page: http://www.synthsys.ed.ac.uk
Andrew Millar's research group, University of Edinburgh
Programme: SynthSys
Public web page: http://www.amillar.org
Data, models and simulations for the Chew et al. 2014 paper (PNAS, https://doi.org/10.1073/pnas.1410238111), using wild-type Arabidopsis ecotype Col-0 in standard 12hL:12hD growth conditions, compared to La(er) or Fei-0 accessions, or to plants overexpressing a micro RNA (miR156).
Submitter: Andrew Millar
Studies: Construction of Framework Model v1, Test of FMv1, growth study of Col-0 accession in 12L:12D, Test of FMv1, growth study of Col-0 accession in 5 photoperiods, Test of FMv1, growth study of other accessions and transgenic line in 12...
Assays: Arabidopsis Framework Model v1, Matlab and Simile version, Gas exchange of Fei-0 and Ler plants in 12hL:12hD, Growth of Col-0 and 35S:miR156 plants in 12hL:12hD, Growth of Col-0 in 12hL:12hD, Growth of Col-0 plants in 5 photoperiods, Growth of Fei-0 and Ler plants in 12hL:12hD
Standard growth conditions and 'wild-type' Arabidopsis accessions other than Col-0 and the 35S:miR156 transgenics, with biomass data for whole rosettes, and in some cases, individual leaf area and leaf biomass data
Submitter: Andrew Millar
Provider Name: Dr. Ronan Sulpice from Max Planck Institute for Molecular Plant Physiology, Potsdam - Golm, Germany
Provider's strain ID: Fei-0
Organism: Arabidopsis thaliana
Genotypes: wild-type
Phenotypes: wild-type
Comment: Accession collected in Santa Maria da Feira village (Portugal)
Data for Figs. 3D, 3E in Chew et al. PNAS 2014 Fresh biomass, dry biomass (i.e. after baking out all water), SLA - specific leaf area (area per g) Also FMv1 model simulation results.
The same data are available on the BioDare resource, with additional experimental meta data on growth conditions. BioDare ID 13790837647786, title "Physiology experiment using Fei", the direct link is: https://www.biodare.ed.ac.uk/robust/ShowExperiment.action?experimentId=13790837647786
Data for Figs. 3A, 3B in Chew et al. PNAS 2014 Fresh biomass, dry biomass (i.e. after baking out all water), SLA - specific leaf area (area per g)
Also contains model simulation data from the FMv1 The same data are available, with additional experimental meta data on growth conditions, from the BioDare resource: BioDare experiment 13790834110003; title "Physiology experiment using Ler", the direct link is: https://www.biodare.ed.ac.uk/robust/ShowExperiment.action?experimentId=13790834110003
Intact rosette is pictured, with plant number and genotype in handwritten labels, and ruler for scale. Then dissected leaves are organised in sequence of age, if necessary with small cuts to let them lie flat. Areas are then measured in image processing.
Data for Figs 3C, 3F and Supp 2 in Chew et al. PNAS 2014 Leaf number of the growing rosettes, from 4 to 37 days after sowing (DAS). Data and also results of FMv1 model simulations. Note that Fei-0 was previously tested by Mendez-Vigo et al, suggesting this line had a higher leaf appearance rate. We suggested that its larger final leaf number was more likely due to faster germination.
